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Rapid Detection and Quantification of Soil Borne Nematodes


Tender Closed
RFx ID : 18851441
Tender Name : Rapid Detection and Quantification of Soil Borne Nematodes
Reference # : 405366
Open Date : Wednesday, 19 July 2017 11:00 AM (Pacific/Auckland UTC+12:00)
Close Date : Wednesday, 16 August 2017 2:00 PM (Pacific/Auckland UTC+12:00)
Department/Business Unit :
Tender Type : Request for Proposals (RFP)
Tender Coverage : Sole Agency  [?]
Categories :
  • 81171800 - Agricultural science services
  • New Zealand
Required Pre-qualifications : None
Contact : Angela Evans
Alternate Physical Delivery Address  :
Alternate Physical Fax Number  :

MPI requires a robust and efficient extraction, identification and quantification method to isolate nematodes from large quantities of soil samples and to extract nematode DNA, in order to determine the presence or absence of unwanted pest nematode species, by PCR or other molecular methods,

The aim of the project is to develop new methodology to allow for the whole diagnostic process from nematode isolation to identification to be completed in around 2 days.

The key outcomes that we want to achieve are to

 Develop a rapid nematode isolation tool
 Develop and validate a DNA extraction tool that allows processing of large numbers of nematodes for molecular detection and quantification of pest nematodes from large volumes of soil samples.

When developed this methodology would enable MPI, associated government agencies, educational institutes, CRI’s, Universities and private companies, to provide timely biosecurity information about possible nematode incursions.